Simple, Rapid, and Selective Isolation of 2S Albumins from Allergenic Seeds and Nuts

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• 作者：Marlene Hummel ; Tina Wigger ; Tessa H枚per ; Imke Westkamp ; Jens Brockmeyer
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：July 8, 2015
• 年：2015
• 卷：63
• 期：26
• 页码：6035-6040
• 全文大小：341K
• ISSN：1520-5118

文摘

The 2S albumins belong to the group of seed storage proteins present in different seeds and nuts. Due to their pronounced allergenic potential, which is often associated with severe allergic reactions, this protein family is of special interest in the field of allergen research. Here we present a simple, rapid, and selective method for the purification of 2S albumins directly from allergenic seeds and nuts. We systematically optimized the parameters 鈥渂uffer system鈥? 鈥渆xtraction temperature鈥? 鈥渂uffer molarity鈥? and 鈥減H 鈥?and were able to achieve 2S albumin purities of about 99% without further purification and demonstrate transferability of this method to nine different allergenic food matrices. Compared to conventional isolation routines, significant reduction of hands-on time and required laboratory equipment is achieved, but nonetheless higher protein yields are obtained. The presented method allows for the rapid purification of different 2S albumins including the corresponding isoforms from natural material.