Mobility-Based Wall Adsorption Isotherms for Comparing Capillary Electrophoresis with Single-Molecule Observations

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• 作者：Ning Fang ; Hui Zhang ; Jiangwei Li ; Hung-Wing Li ; Edward S. Yeung
• 刊名：Analytical Chemistry
• 出版年：2007
• 出版时间：August 15, 2007
• 年：2007
• 卷：79
• 期：16
• 页码：6047 - 6054
• 全文大小：190K
• 年卷期：v.79,no.16(August 15, 2007)
• ISSN：1520-6882

文摘

The adsorption properties of R-phycoerythrin (RPE), anautofluorescent protein, on the fused-silica surface werestudied in capillary electrophoresis (CE) and in single-molecule experiments. The band shapes and migrationtimes were measured in CE, and adsorption and desorption events were recorded at the single-molecule level byimaging within the evanescent field layer using totalinternal reflection fluorescence microscopy. The adsorbedRPE molecules on the surface of the fused-silica prismwere counted with confidence based on ImageJ software.The capacity factor and desorption rate were estimatedfrom the counting results. The mobility-based adsorptionisotherms were constructed from both computer simulations and experiments to determine the capacity factor.