On-Line Combination of High Performance Liquid Chromatography with Comprehensive Two-Dimensional Gas Chromatography-Triple Quadrupole Mass Spectrometry: A Proof of Principle Study

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• 作者：Mariosimone Zoccali ; Peter Quinto Tranchida ; Luigi Mondello
• 刊名：Analytical Chemistry
• 出版年：2015
• 出版时间：February 3, 2015
• 年：2015
• 卷：87
• 期：3
• 页码：1911-1918
• 全文大小：370K
• ISSN：1520-6882

文摘

The present contribution is focused on the on-line combination of high performance liquid chromatography (HPLC), cryogenically modulated comprehensive two-dimensional gas chromatography (GC 脳 GC), and triple quadrupole mass spectrometry (QqQ MS), generating a very powerful unified separation-science tool. The instrument can be used in seven different combinations ranging from one-dimensional HPLC with a photodiode array detector to on-line LC 脳 GC 脳 GC/QqQ MS. The main focus of the present research is directed to the LC-GC 脳 GC/QqQ MS configuration, with its analytical potential shown in a proof-of-principle study involving a very complex sample, namely, coal tar. Specifically, a normal-phase LC process enabled the separation of three classes of coal tar compounds: (1) nonaromatic hydrocarbons; (2) unsaturated compounds (with and without S); (3) oxygenated constituents. The HPLC fractions were transferred to the GC 脳 GC instrument via a syringe-based interface mounted on an autosampler. Each fraction was subjected to a specific programmed temperature vaporizer GC 脳 GC/QqQ MS untargeted or targeted analysis. For example, the coal tar S-containing compounds were pinpointed through multiple-reaction-monitoring analysis, while full-scan information was attained for the oxygenated constituents.