Facile Synthesis of Monodispersed Mesoporous Silica Nanoparticles with Ultralarge Pores and Their Application in Gene Delivery

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• 作者：Mi-Hee Kim ; Hee-Kyung Na ; Young-Kwan Kim ; Soo-Ryoon Ryoo ; Hae Sung Cho ; Kyung Eun Lee ; Hyesung Jeon ; Ryong Ryoo ; Dal-Hee Min
• 刊名：ACS Nano
• 出版年：2011
• 出版时间：May 24, 2011
• 年：2011
• 卷：5
• 期：5
• 页码：3568-3576
• 全文大小：1101K
• 年卷期：0
• ISSN：1936-086X

文摘

Among various nanoparticles, the silica nanoparticle (SiNP) is an attractive candidate as a gene delivery carrier due to advantages such as availability in porous forms for encapsulation of drugs and genes, large surface area to load biomacromolecules, biocompatibility, storage stability, and easy preparation in large quantity with low cost. Here, we report on a facile synthesis of monodispersed mesoporous silica nanoparticles (MMSN) possessing very large pores (>15 nm) and application of the nanoparticles to plasmid DNA delivery to human cells. The aminated MMSN with large pores provided a higher loading capacity for plasmids than those with small pores (∼2 nm), and the complex of MMSN with plasmid DNA readily entered into cells without supplementary polymers such as cationic dendrimers. Furthermore, MMSN with large pores could efficiently protect plasmids from nuclease-mediated degradation and showed much higher transfection efficiency of the plasmids encoding luciferase and green fluorescent protein (pLuc, pGFP) compared to MMSN with small pores (∼2 nm).