Maltogenic amylase and
-glucanotransferase (
-GTase) were employed in an effort to develop anefficient process for the production of isomaltooligosaccharides (IMOs).
Bacillus stearothermophilusmaltogenic amylase (BSMA) and
-GTase from
Thermotoga maritima were overexpressed in
Escherichia coli using overexpression vectors. An IMO mixture containing 58% of various IMOs wasproduced from liquefied corn syrup by the hydrolyzing and transglycosylation activities of BSMA alone.When BSMA and
-GTase were reacted simultaneously, the IMO content increased to 68% andcontained relatively larger IMOs compared with the products obtained by the reaction without
-GTase.Time course analysis of the IMO production suggested that BSMA hydrolyzed maltopentaose andmaltohexaose most favorably into maltose and maltotriose and transferred the resulting moleculessimultaneously to acceptor molecules to form IMOs.
-GTase transferred donor sugar molecules tothe hydrolysis products such as maltose and maltotriose to form maltopentaose, which was thenrehydrolyzed by BSMA as a favorable substrate.Keywords:
Bacillus stearothermophilus maltogenic amylase; isomaltooligosaccharides (IMOs);
Thermotoga maritima;
-glucanotransferase (
-GTase)