A Mitochondrial-Targeted Two-Photon Probe for Zinc Ion

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• 作者：Goutam Masanta ; Chang Su Lim ; Hyung Joong Kim ; Ji Hee Han ; Hwan Myung Kim ; Bong Rae Cho
• 刊名：Journal of the American Chemical Society
• 出版年：2011
• 出版时间：April 20, 2011
• 年：2011
• 卷：133
• 期：15
• 页码：5698-5700
• 全文大小：750K
• 年卷期：v.133,no.15(April 20, 2011)
• ISSN：1520-5126

文摘

We report a two-photon probe (SZn-Mito) for mitochondrial zinc ions ([Zn²⁺]_m). This probe shows a 7-fold enhancement of two-photon-excited fluorescence in response to Zn²⁺ with a dissociation constant (K_d^TP) of 3.1 卤 0.1 nM and pH insensitivity in the biologically relevant range, allowing the detection of [Zn²⁺]_m in a rat hippocampal slice at a depth of 100鈭?00 渭m without interference from other metal ions through the use of two-photon microscopy.