文摘
Light-induced cell detachment shows much potential in <i>in vitroi> cell culture and calls for high-performance light-responsive films. In this study, a smooth and dense SiO<sub>2sub>/TiO<sub>2sub> nanocomposite thin film with thickness of around 250 nm was first fabricated on H<sub>2sub>O<sub>2sub> treated polystyrene (PS) substrate via a low-temperature sol–gel method. It was observed that the film could well-adhere on the PS surface and the bonding strength became increasingly high with the increase of SiO<sub>2sub> content. The peeling strength and shear strength reached 3.05 and 30.02 MPa, respectively. It was observed the surface of the film could transform into superhydrophilic upon 20 min illumination of ultraviolet with a wavelength of 365 nm (UV365). In cell culture, cells, <i>i.e.i>, NIH3T3 and MC3T3-E1 cells, cultured on SiO<sub>2sub>/TiO<sub>2sub> nanocomposite film were easily detached after 10 min of UV365 illumination; the detachment rates reached 90.8% and 88.6%, respectively. Correspondingly, continuous cell sheets with good viability were also easily obtained through the same way. The present work shows that SiO<sub>2sub>/TiO<sub>2sub> nanocomposite thin film could be easily prepared on polymeric surface at low temperature. The corresponding film exhibits excellent biocompatibility, high bonding strength, and good light responses. It could be a good candidate for the surface of cell culture utensils with light-induced cell detachment property.