Use of Near-Infrared Spectroscopy for Screening the Individual and Total Glucosinolate Contents in Indian Mustard Seed (Brassica juncea L. Czern. & Coss.)
详细信息 查看全文
- 作者：Rafael Font ; Mercedes del Rí ; o ; José ; M. Ferná ; ndez-Martí ; nez ; and Antonio de Haro-Bailó ; n
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2004
- 出版时间：June 2, 2004
- 年：2004
- 卷：52
- 期：11
- 页码：3563 - 3569
- 全文大小：123K
- 年卷期：v.52,no.11(June 2, 2004)
- ISSN：1520-5118
文摘
The potential of near-infrared spectroscopy (NIRS) for screening the sinigrin, gluconapin, 4-hydroxyglucobrassicin, and total glucosinolate contents of Indian mustard (Brassica juncea L. Czern. & Coss.)seed was assessed. Intact seed samples of this species were analyzed by NIRS and their referencevalues regressed against different spectral transformations by modified partial least-squares (MPLS)regression. The coefficients of determination (r 2) for sinigrin, gluconapin, 4-hydroxyglucobrassicin,and total glucosinolate contents were, respectively, 0.86, 0.95, 0.33, and 0.82. The standard deviationto standard error of prediction (SEP) ratio, and SEP to standard error of laboratory ratio were forthese constituents as follows: sinigrin, 2.59 and 2.70; gluconapin, 4.16 and 2.08; 4-hydroxyglucobrassicin, 1.18 and 1.40; and total glucosinolates, 2.18 and 1.60. By comparison of commercial sinigrinspectrum with the first MPLS loadings of the sinigrin equation, it can be concluded that the moleculeof sinigrin has a specific signal in the seed spectrum of Brassica.Keywords: Near-infrared spectroscopy; screening; Brassica juncea; glucosinolates; agriculture;biofumigants