Chromatographic Determination of Fe Chelated by Ethylenediamine-N-(o-hydroxyphenylacetic)-N'-(p-hydroxyphenylacetic) Acid in Commercial EDDHA/Fe3+ Fertilizers

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• 作者：Sonia Garc&iacute ; a-Marco ; Ana Torreblanca ; and Juan J. Lucena
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2006
• 出版时间：February 22, 2006
• 年：2006
• 卷：54
• 期：4
• 页码：1380 - 1386
• 全文大小：94K
• 年卷期：v.54,no.4(February 22, 2006)
• ISSN：1520-5118

文摘

EDDHA/Fe³⁺ chelates are the most common fertilizers used to solve Fe chlorosis in establishedcrops. Commercial products contain two regioisomers, ethylenediamine-N,N'-bis(o-hydroxyphenylacetic) acid (o,o-EDDHA)/Fe³⁺ and ethylenediamine-N-(o-hydroxyphenylacetic)-N'-(p-hydroxyphenylacetic) acid (o,p-EDDHA)/Fe³⁺. Although several chromatographic methods exist for the determination of Fe³⁺ chelated by the o,o-EDDHA isomer, no method has been described for thequantification of Fe³⁺ chelated by o,p-EDDHA. In this work, factors that affect the behavior of o,p-EDDHA/Fe³⁺ in ion pair chromatography are reviewed: pH, ion pair reagent, and organic modifier.The best chromatographic performance was obtained with an aqueous mobile phase at pH 6.0containing 35% acetonitrile and 5 mM tetrabutylammonium hydroxide under isocratic elution conditions.This method was applied to the quantification of commercial samples.Keywords: EDDHA/Fe³⁺; Fe³⁺ chelates; fertilizers; HPLC; o,o-EDDHA/Fe³⁺; o,p-EDDHA/Fe³⁺