Total Oxidant Scavenging Capacity of Euterpe oleracea Mart. (Açaí) Seeds and Identification of Their Polyphenolic Compounds

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• 作者：Roberta B. Rodrigues ; Ramona Lichtenthä ; ler ; Benno F. Zimmermann ; Menelaos Papagiannopoulos ; Heinz Fabricius ; Friedhelm Marx ; José ; G. S. Maia ; and Ossalin Almeida
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2006
• 出版时间：June 14, 2006
• 年：2006
• 卷：54
• 期：12
• 页码：4162 - 4167
• 全文大小：117K
• 年卷期：v.54,no.12(June 14, 2006)
• ISSN：1520-5118

文摘

The antioxidant capacity of methanol and ethanol seed extracts from Euterpe oleracea Mart. (açaí)against the reactive oxygen species (ROS) peroxyl radicals, peroxynitrite, and hydroxyl radicals wasstudied with the total oxidant scavenging capacity (TOSC) assay in a modified and automated version.Cold methanol digestion was the most efficient extraction method with respect to the antioxidantcapacity. The extracts exhibit good antioxidant capacity against peroxyl radicals, similar to the capacityof the pulp. The antioxidant capacity against peroxynitrite and hydroxyl radicals is even higher. Themain antioxidants identified by HPLC-MS and HPLC-CEAD are five different procyanidins (di- throughpentamers); furthermore, protocatechuic acid and epicatechin were identified as minor compounds.Determination of TOSC values of HPLC seed extract fractions indicates that the procyanidins contributesubstantially to the overall antioxidant capacity. In addition, however, other compounds that havenot yet been identified are responsible for a large part of the observed antioxidant capacity.Keywords: Euterpe oleracea; açaí; seed extract; TOSC assay; antioxidant; peroxyl radicals; peroxynitrite; hydroxyl radicals; procyanidins