Rhamnolipid Biosurfactant Production by Strains of Pseudomonas aeruginosa Using Low-Cost Raw Materials

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• 作者：K. S. M. Rahman ; Thahira J. Rahman ; Stephen McClean ; Roger Marchant ; and Ibrahim M. Banat
• 刊名：Biotechnology Progress
• 出版年：2002
• 出版时间：December 2002
• 年：2002
• 卷：18
• 期：6
• 页码：1277 - 1281
• 全文大小：101K
• 年卷期：v.18,no.6(December 2002)
• ISSN：1520-6033

文摘

This study was aimed at the development of economical methods for higher yields ofbiosurfactant by suggesting the use of low-cost raw materials. Two oil-degradingstrains, Pseudomonas aeruginosa GS9-119 and DS10-129, were used to optimize asubstrate for maximum rhamnolipid production. Among the two strains, the latterproduced maxima of 4.31, 2.98, and 1.77 g/L rhamnolipid biosurfactant using soybeanoil, safflower oil, and glycerol, respectively. The yield of biosurfactant steadily increasedeven after the bacterial cultures reached the stationary phase of growth. Characterization of rhamnolipids using mass spectrometry revealed the presence of dirhamnolipids(Rha-Rha-C₁₀-C₁₀). Emulsification activity of the rhamnolipid biosurfactant producedby P. aeruginosa DS10-129 was greater than 70% using all the hydrocarbons tested,including xylene, benzene, hexane, crude oil, kerosene, gasoline, and diesel. P.aeruginosa GS9-119 emulsified only hexane and kerosene to that level.