Geometrical Separation Method for Lipoproteins Using Bioformulated-Fiber Matrix Electrophoresis: Size of High-Density Lipoprotein Does Not Reflect Its Density

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• 作者：Mari Tabuchi ; Makoto Seo ; Takayuki Inoue ; Takeshi Ikeda ; Akinori Kogure ; Ikuo Inoue ; Shigehiro Katayama ; Toshiyuki Matsunaga ; Akira Hara ; Tsugikazu Komoda
• 刊名：Analytical Chemistry
• 出版年：2011
• 出版时间：February 1, 2011
• 年：2011
• 卷：83
• 期：3
• 页码：1131-1136
• 全文大小：852K
• 年卷期：v.83,no.3(February 1, 2011)
• ISSN：1520-6882

文摘

The increasing number of patients with metabolic syndrome is a critical global problem. In this study, we describe a novel geometrical electrophoretic separation method using a bioformulated-fiber matrix to analyze high-density lipoprotein (HDL) particles. HDL particles are generally considered to be a beneficial component of the cholesterol fraction. Conventional electrophoresis is widely used but is not necessarily suitable for analyzing HDL particles. Furthermore, a higher HDL density is generally believed to correlate with a smaller particle size. Here, we use a novel geometrical separation technique incorporating recently developed nanotechnology (Nata de Coco) to contradict this belief. A dyslipidemia patient given a 1-month treatment of fenofibrate showed an inverse relationship between HDL density and size. Direct microscopic observation and morphological observation of fractionated HDL particles confirmed a lack of relationship between particle density and size. This new technique may improve diagnostic accuracy and medical treatment for lipid related diseases.