A Microfluidic Device with Integrated Sonication and Immunoprecipitation for Sensitive Epigenetic Assays
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- 作者：Zhenning Cao ; Chang Lu
- 刊名：Analytical Chemistry
- 出版年：2016
- 出版时间：February 2, 2016
- 年：2016
- 卷：88
- 期：3
- 页码：1965-1972
- 全文大小：414K
- ISSN：1520-6882
文摘
Epigenetic studies increasingly require analysis of a small number of cells that are of one specific type and derived from patients or animals. In this report, we demonstrate a simple microfluidic device that integrates sonication and immunoprecipitation (IP) for epigenetic assays, such as chromatin immunoprecipitation (ChIP) and methylated DNA immunoprecipitation (MeDIP). By incorporating an ultrasonic transducer with a microfluidic chamber, we implemented microscale sonication for both shearing chromatin/DNA and mixing/washing of IP beads. Such integration allowed highly sensitive tests starting with 100 cross-linked cells for ChIP or 500 pg of genomic DNA for MeDIP (compared to 106–107 cells for ChIP and 1–10 μg of DNA for MeDIP in conventional assays). The entire on-chip process of sonication and IP took only 1 h. Our tool will be useful for highly sensitive epigenetic studies based on a small quantity of sample.