Potassium−Lead-Switched G-Quadruplexes: A New Class of DNA Logic Gates

详细信息    查看全文

• 作者：Tao Li ; Erkang Wang ; Shaojun Dong
• 刊名：Journal of the American Chemical Society
• 出版年：2009
• 出版时间：October 28, 2009
• 年：2009
• 卷：131
• 期：42
• 页码：15082-15083
• 全文大小：164K
• 年卷期：v.131,no.42(October 28, 2009)
• ISSN：1520-5126

文摘

A cation-driven allosteric G-quadruplex DNAzyme (PW17) was utilized to devise a conceptually new class of DNA logic gate based on cation-tuned ligand binding and release. K⁺ favors the binding of hemin to parallel-stranded PW17, thereby promoting the DNAzyme activity, whereas Pb²⁺ induces PW17 to undergo a parallel-to-antiparallel conformation transition and thus drives hemin to release from the G-quadruplex, deactivating the DNAzyme. Such a K⁺−Pb²⁺ switched G-quadruplex, in fact, functions as a two-input INHIBIT logic gate. With the introduction of another input EDTA, this G-quadruplex can be further utilized to construct a reversibly operated IMPLICATION gate.