Greek Plant Extracts Exhibit Selective Estrogen Receptor Modulator (SERM)-like Properties
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- 作者：Eva Kassi ; Zoi Papoutsi ; Nikolaos Fokialakis ; Ioanna Messari ; Sophia Mitakou ; and Paraskevi Moutsatsou
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2004
- 出版时间：November 17, 2004
- 年：2004
- 卷：52
- 期：23
- 页码：6956 - 6961
- 全文大小：305K
- 年卷期：v.52,no.23(November 17, 2004)
- ISSN：1520-5118
文摘
To prevent bone loss that occurs with increasing age, nutritional and pharmacological factors areneeded. Traditional therapeutic agents (selective estrogen receptor modulators or SERMs, biphosphonates, calcitonin) may have serious side effects or contraindications. In an attempt to find foodcomponents potentially acting as SERMs, we submitted four plant aqueous extracts derived fromGreek flora (Sideritis euboea, Sideritis clandestina, Marticaria chamomilla, and Pimpinella anisum)in a series of in vitro biological assays reflective of SERM profile. We examined their ability (a) tostimulate the differentiation and mineralization of osteoblastic cell culture by histochemical stainingfor alkaline phosphatase and Alizarin Red-S staining, (b) to induce, like antiestrogens, the insulingrowth factor binding protein 3 (IGFBP3) in MCF-7 breast cancer cells, and (c) to proliferate cervicaladenocarcinoma (HeLa) cells by use of MTT assay. Our data reveal that all the plant extracts studiedat a concentration range 10-100 
g/mL stimulate osteoblastic cell differentiation and exhibitantiestrogenic effect on breast cancer cells without proliferative effects on cervical adenocarcinomacells. The presence of estradiol inhibited the antiestrogenic effect induced by the extracts on MCF-7cells, suggesting an estrogen receptor-related mechanism. In conclusion, the aqueous extracts derivedfrom Sideritis euboea, Sideritis clandestina, Marticaria chamomilla, and Pimpinella anisum may formthe basis to design "functional foods" for the prevention of osteoporosis.Keywords: Sideritis; Anisum; Chamomilla; selective estrogen receptor modulators; plant extracts
g/mL stimulate osteoblastic cell differentiation and exhibitantiestrogenic effect on breast cancer cells without proliferative effects on cervical adenocarcinomacells. The presence of estradiol inhibited the antiestrogenic effect induced by the extracts on MCF-7cells, suggesting an estrogen receptor-related mechanism. In conclusion, the aqueous extracts derivedfrom Sideritis euboea, Sideritis clandestina, Marticaria chamomilla, and Pimpinella anisum may formthe basis to design "functional foods" for the prevention of osteoporosis.Keywords: Sideritis; Anisum; Chamomilla; selective estrogen receptor modulators; plant extracts