Quantitation of Mule Duck in Goose Foie Gras Using TaqMan Real-Time Polymerase Chain Reaction

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• 作者：Miguel A. Rodrí ; guez ; Teresa Garcí ; a ; Isabel Gonz&aacute ; lez ; Luis Asensio ; Pablo E. Hern&aacute ; ndez ; and Rosario Martí ; n
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2004
• 出版时间：March 24, 2004
• 年：2004
• 卷：52
• 期：6
• 页码：1478 - 1483
• 全文大小：114K
• 年卷期：v.52,no.6(March 24, 2004)
• ISSN：1520-5118

文摘

A real-time quantitative Polymerase Chain Reaction (PCR) method has been developed for thequantitation of mule duck (Anas platyrhynchos × Cairina moschata) in binary duck/goose foie grasmixtures. The method combines the use of real-time PCR with duck-specific and endogenous control"duck + goose" primers to measure duck content and total foie gras content, respectively. Both PCRsystems (duck-specific and duck + goose) were designed on the mitochondrial 12S ribosomal RNAgene (rRNA). The duck-specific system amplifies a 96 bp fragment from duck DNA, whereas theduck + goose system amplifies a 120 bp fragment from duck and goose DNA. The method measuresPCR product accumulation through a FAM-labeled fluorogenic probe (TaqMan). The C_t (thresholdcycle) values obtained from the duck + goose system are used to normalize the ones obtained fromthe duck-specific system. Analysis of experimental duck/goose foie gras binary mixtures demonstratedthe suitability of the assay for the detection and quantitation of duck in the range of 1-25%. Thisgenetic marker can be very useful to avoid mislabeling or fraudulent species substitution of goose byduck in foie gras.Keywords: Species identification; 12S rRNA; foie gras; real-time PCR