Speeding Up 13C Direct Detection Biomolecular NMR Spectroscopy

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• 作者：Wolfgang Bermel ; Ivano Bertini ; Isabella C. Felli ; Roberta Pierattelli
• 刊名：Journal of the American Chemical Society
• 出版年：2009
• 出版时间：October 28, 2009
• 年：2009
• 卷：131
• 期：42
• 页码：15339-15345
• 全文大小：228K
• 年卷期：v.131,no.42(October 28, 2009)
• ISSN：1520-5126

文摘

After the exploitation of ¹H polarization as a starting source for ¹³C direct detection experiments, pulse sequences are designed which exploit the accelerated ¹H longitudinal relaxation to expedite ¹³C direct detection experiments. We show here that 2D experiments based on ¹³C direct detection on a 0.5 mM water sample of ubiquitin can be recorded in a few minutes and 3D experiments in a few hours. We also show that fast methods like nonuniform sampling can be easily implemented. As overall experimental time has always been a counter indication for the use of ¹³C direct detection experiments, this research opens new avenues for the application of ¹³C NMR to biological molecules.