Food-Derived Peptides Stimulate Mucin Secretion and Gene Expression in Intestinal Cells
详细信息 查看全文
- 作者：Daniel Mart铆nez-Maqueda ; Beatriz Miralles ; Sonia De Pascual-Teresa ; In茅s Rever贸n ; Rosario Mu帽oz ; Isidra Recio
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2012
- 出版时间：September 5, 2012
- 年：2012
- 卷：60
- 期：35
- 页码：8600-8605
- 全文大小：227K
- 年卷期：v.60,no.35(September 5, 2012)
- ISSN：1520-5118
文摘
In this study, the hypothesis that food-derived opioid peptides besides 尾-casomorphin 7 might modulate the production of mucin via a direct action on epithelial goblet cells was investigated in HT29-MTX cells used as a model of human colonic epithelium. Seven milk whey or casein peptides, a human milk peptide, and a wheat gluten-derived peptide with proved or probable ability to bind 渭- or 未-opioid receptors were tested on the cell culture. Significantly increased secretion of mucins was found after exposure to six of the assayed peptides, besides the previously described 尾-casomorphin 7, as measured by an enzyme-linked lectin assay (ELLA). Human 尾-casomorphin 5 and 伪-lactorphin were selected to study the expression of mucin 5AC gene (MUC5AC), the HT29-MTX major secreted mucin gene. 伪-Lactorphin showed increased expression of MUC5AC from 4 to 24 h (up to 1.6-fold over basal level expression), although differences were statistically different only after 24 h of exposure. However, this increased expression of MUC5AC did not reach significance after cell treatment with human 尾-casomorphin 5. In conclusion, six food-derived peptides have been identifed with described or probable opioid activity that induce mucin secretion in HT29-MTX cells. Concretely, 伪-lactorphin is able to up-regulate the expression of the major secreted mucin gene encoded by these cells.