Self-Supporting Hydrogel Stamps for the Microcontact Printing of Proteins

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• 作者：Na&iuml ; s Coq ; Ties van Bommel ; Rifat A. Hikmet ; Hendrik R. Stapert ; Wendy U. Dittmer
• 刊名：Langmuir
• 出版年：2007
• 出版时间：April 24, 2007
• 年：2007
• 卷：23
• 期：9
• 页码：5154 - 5160
• 全文大小：444K
• 年卷期：v.23,no.9(April 24, 2007)
• ISSN：1520-5827

文摘

In this work we explore a new hydrogel stamp material obtained from polymerizing 2-hydroxyethyl acrylate andpoly(ethylene glycol) diacrylate in the presence of water for the microcontact printing of proteins directly on goldsubstrates and by covalent coupling to self-assembled monolayers of alkanethiols. At high cross-link density, thehydrogel is rigid, hydrophilic, and with a high buffer holding capacity to enable the unsupported printing of proteinpatterns homogeneously and reproducibly, with micrometer-range precision. The stamps were used to print antibodiesto human parathyroid hormone, which were shown using immunoassay tests to retain their biological function withbinding capacities comparable to those of solution-adsorbed antibodies.