Stably transformed transgenic hairy root cultures have the potential to be a valuable productionplatform for a variety of secondary metabolites. This study reports that a transgenic hairy rootculture of Catharanthus roseus has been stably maintained for over 4.5 years. This culture carriesa transgene that expresses the green fluorescent protein under the control of the glucocorticoid-inducible promoter. Genomic PCR confirmed the presence of the GFP insert within the hairyroots, and induction with dexamethasone caused a significant (p < 0.02) increase in GFP levels.