Altering the Communication Networks of Multispecies Microbial Systems Using a Diverse Toolbox of AI-2 Analogues

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• 作者：Sonja Gamby ; Varnika Roy ; Min Guo ; Jacqueline A. I. Smith ; Jingxin Wang ; Jessica E. Stewart ; Xiao Wang ; William E. Bentley ; Herman O. Sintim
• 刊名：ACS Chemical Biology
• 出版年：2012
• 出版时间：June 15, 2012
• 年：2012
• 卷：7
• 期：6
• 页码：1023-1030
• 全文大小：567K
• 年卷期：v.7,no.6(June 15, 2012)
• ISSN：1554-8937

文摘

There have been intensive efforts to find small molecule antagonists for bacterial quorum sensing (QS) mediated by the 鈥渦niversal鈥?QS autoinducer, AI-2. Previous work has shown that linear and branched acyl analogues of AI-2 can selectively modulate AI-2 signaling in bacteria. Additionally, LsrK-dependent phosphorylated analogues have been implicated as the active inhibitory form against AI-2 signaling. We used these observations to synthesize an expanded and diverse array of AI-2 analogues, which included aromatic as well as cyclic C-1-alkyl analogues. Species-specific analogues that disrupted AI-2 signaling in Escherichia coli and Salmonella typhimurium were identified. Similarly, analogues that disrupted QS behaviors in Pseudomonas aeruginosa were found. Moreover, we observed a strong correlation between LsrK-dependent phosphorylation of these acyl analogues and their ability to suppress QS. Significantly, we demonstrate that these analogues can selectively antagonize QS in single bacterial strains in a physiologically relevant polymicrobial culture.