Polymeric Optical Microscreen for High-Resolution Surface Plasmon Resonance Microarray Imaging

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• 作者：Jaeyeon Jung ; Jong Seol Yuk ; Kyunga Na ; Okgene Kim ; Jonghwan Lee ; Kyusik Yun ; Kwon-Soo Ha ; Jinho Hyun
• 刊名：Langmuir
• 出版年：2007
• 出版时间：September 25, 2007
• 年：2007
• 卷：23
• 期：20
• 页码：10403 - 10406
• 全文大小：222K
• 年卷期：v.23,no.20(September 25, 2007)
• ISSN：1520-5827

文摘

In this study, we demonstrate a simple method to fabricate surface plasmon resonance (SPR) imaging microarraysusing polymer micropatterns. The use of a micrometer-scale polymeric optical screen (microPOS) passivates the regiondeposited with polymer by completely removing SPR signals or by saturating the SPR signal far beyond the detectionrange of SPR imaging. Two schemes were suggested to create a surface microPOS by either micropatterning a thickinsulating layer before deposition of a metal layer (complete removal of SPR) or after deposition of a metal layer(saturation of SPR signal). The two schemes were successfully applied for the imaging of biological adsorption witha high imaging resolution of ~100 m/pattern and 10 m separation. The validity of the system was verified witha biotin-streptavidin system as a model for the systematic binding of biomolecules. Further, binding of prostate-specific antigen (PSA) onto the anti-PSA SPR microarray was demonstrated as a useful method for detecting a cancermarker.