"Smart" Biopolymer for a Reversible Stimuli-Responsive Platform in Cell-Based Biochips
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- 作者：Kyunga Na ; Jaeyeon Jung ; Okgene Kim ; Jonghwan Lee ; Tae Geol Lee ; Young Hwan Park ; Jinho Hyun
- 刊名：Langmuir
- 出版年：2008
- 出版时间：May 6, 2008
- 年：2008
- 卷：24
- 期：9
- 页码：4917 - 4923
- 全文大小：593K
- 年卷期：v.24,no.9(May 6, 2008)
- ISSN：1520-5827
文摘
The rapid response of a smart material surface to external stimuli is critical for application to cell-based biochips.The sharp and controllable phase transition of elastin-like polypeptide (ELP) enabled reversible cell adhesion on thesurface by changing the temperature or salt concentration in the system. First, ELP micropatterns were prepared ona glass surface modified into aldehyde. The lysine-containing ELP (ELP-K) was genetically synthesized from E. colifor conjugation with the aldehyde on the glass surface. The phase transition of ELP was monitored in PBS and cellculture media using UV-visible spectroscopy, and a significant difference in transition temperature (Tt) was observedbetween the two solution systems. The micropatterning of ELP on the glass surface was performed by microcontactprinting a removable polymeric template on the aldehyde-glass followed by incubation in ELP-K aqueous solution.The ELP micropatterns were imaged with atomic force microscopy and showed a monolayer thickness of ~4 nm.Imaging from time-of-flight secondary ion mass spectroscopy confirmed that the ELP molecules were successfullyimmobilized on the highly resolved micropatterns. Cell attachment and detachment could be reversibly controlled onthe ELP surfaces by external stimuli. The hydrophobic phase above Tt resulted in the adhesion of fibroblasts, whilethe detachment of cells was induced by lowering the incubation temperature below Tt. The smart properties of ELPwere reliable and reproducible, demonstrating potential applications in cell-based microdevices.