Study of Langmuir and Langmuir-Blodgett Films of Odorant-Binding Protein/Amphiphile for Odorant Biosensors

详细信息    查看全文

• 作者：Yanxia Hou ; Nicole Jaffrezic-Renault ; Claude Martelet ; Chaker Tlili ; Aidong Zhang ; Jean-Claude Pernollet ; Loï ; c Briand ; Gabriel Gomila ; Abdelhamid Errachid ; Josep Samitier ; Ludovic Salvagnac ; Beno
• 刊名：Langmuir
• 出版年：2005
• 出版时间：April 26, 2005
• 年：2005
• 卷：21
• 期：9
• 页码：4058 - 4065
• 全文大小：544K
• 年卷期：v.21,no.9(April 26, 2005)
• ISSN：1520-5827

文摘

To make ultrathin films for the fabrication of artificial olfactory systems, odorant biosensors, we haveinvestigated mixed Langmuir and Langmuir-Blodgett films of odorant-binding protein/amphiphile. Underoptimized experimental conditions (phosphate buffer solution, pH 7.5, OBP-1F concentration of 4 mg L^-1,target pressure 35 mN m^-1), the mixed monolayer at the air/water interface is very stable and has beenefficiently transferred onto gold supports, which were previously functionalized by self-assembled monolayers(SAMs) with 1-octadecanethiol (ODT). Atomic force microscopy and electrochemical impedance spectroscopywere used to characterize mixed Langmuir-Blodgett (LB) films before and after contact with a specificodorant molecule, isoamyl acetate. AFM phase images show a higher contrast after contact with theodorant molecule due to the new structure of the OBP-1F/ODA LB film. Non-Faradaic electrochemicalspectroscopy (EIS) is used to quantify the effect of the odorant based on the electrical properties of theOBP-1F/ODA LB film, as its resistance strongly decreases from 1.18 M (before contact) to 25 k (aftercontact).