Sensitivity and Specificity Improvement of an Ion Sensitive Field Effect Transistors-Based Biosensor for Potato Glycoalkaloids Detection
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- 作者：Yaroslav I. Korpan ; Frank M. Raushel ; Elena A. Nazarenko ; Alexey P. Soldatkin ; Nicole Jaffrezic-Renault ; and Claude Martelet
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2006
- 出版时间：February 8, 2006
- 年：2006
- 卷：54
- 期：3
- 页码：707 - 712
- 全文大小：219K
- 年卷期：v.54,no.3(February 8, 2006)
- ISSN：1520-5118
文摘
Butyryl cholinesterase of different origin along with variations of the time of enzyme immobilizationon the potentiometric transducer surface is offered to control the ion sensitive field effect transistor(ISFET)-based biosensor sensitivity. Because butyryl cholinesterase has been already used to developthe sensors for heavy metals, organophosphorus/carbamate pesticides, and steroidal glycoalkaloidsanalysis, the present study has been focused on the investigation and adjustment of the ISFET-based biosensor specificity exclusively to the glycoalkaloids. Utilization of ethylendiaminetetracetate(a complexon of heavy metal ions) and phosphotriesterase (a highly efficient catalyst for the hydrolysisof organophosphorus compounds) enabled the highly specific determination of glycoalkaloids at thebackground of lead and mercury (up to 500 
M of ions concentration) and paraoxon (up to 100 Mof pesticide concentration). The developed biosensor has been validated for glycoalkaloids detectionin potato varieties cultivated in Ukraine, and the results obtained are compared to those measuredby the methods of HPLC and TLC.Keywords: ISFET-based biosensor; sensitivity/specificity improvement; potato glycoalkaloids; phosphotriesterase; butyryl cholinesterase
M of ions concentration) and paraoxon (up to 100 Mof pesticide concentration). The developed biosensor has been validated for glycoalkaloids detectionin potato varieties cultivated in Ukraine, and the results obtained are compared to those measuredby the methods of HPLC and TLC.Keywords: ISFET-based biosensor; sensitivity/specificity improvement; potato glycoalkaloids; phosphotriesterase; butyryl cholinesterase