Reversed-Phase HPLC Separation of Water-Soluble, Monolayer-Protected Quantum Dots
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- 作者：Stephanie A. Owens ; Margaret C. Carpenter ; James W. H. Sonne ; Corwin A. Miller ; Jack R. Renehan ; Charles A. Odonkor ; Elizabeth M. Henry ; Deon T. Miles
- 刊名：Journal of Physical Chemistry C
- 出版年：2011
- 出版时间：October 6, 2011
- 年：2011
- 卷：115
- 期：39
- 页码：18952-18957
- 全文大小：802K
- 年卷期：v.115,no.39(October 6, 2011)
- ISSN：1932-7455
文摘
The synthesis and characterization of water-soluble, monolayer-protected CdSe and ZnSe quantum dots (QDs) is described. The QDs were prepared using various water-soluble thiol ligands in different metal-to-thiol ratios. This work presents use of reversed-phase, high-performance liquid chromatography (RP-HPLC) to separate QDs of different sizes. Polydisperse QD samples were separated into monodisperse fractions using RP-HPLC, with resulting fwhm values <12 nm. The separation is based on the polarity of the surrounding thiol as well as the size of the nanoparticle. In addition, the pH behavior of the water-soluble QDs was studied. The QDs appear to be most stable in basic solutions and are best preserved over long periods of time when dried.