Functionalized Tricarbocyanine Dyes as Near-Infrared Fluorescent Probes for Biomolecules

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• 作者：James H. Flanagan Jr. ; Shaheer H. Khan ; Steve Menchen ; Steven A. Soper ; and Robert P. Hammer
• 刊名：Bioconjugate Chemistry
• 出版年：1997
• 出版时间：September 1997
• 年：1997
• 卷：8
• 期：5
• 页码：751 - 756
• 全文大小：203K
• 年卷期：v.8,no.5(September 1997)
• ISSN：1520-4812

文摘

The syntheses of three novel functionalized tricarbocyanine dyes aredescribed. These dyes containingisothiocyanate and succinimidyl ester functional groups are reactivetoward primary amines and canbe used as fluorescent probes for biologically pertinent compounds suchas amino acids andfunctionalized dideoxynucleotides. The absorption and fluorescencemaxima occur in the near-IR regionof the spectrum (770-810 nm). The succinimidyl ester proved tobe very sensitive to hydrolysis andwas generated in situ to label amino acids. Theisothiocyanates were less susceptible to hydrolysisand were conjugated using organic modified [40% (v/v) acetonitrile]buffers to amino acids. A dyewith an alkyl isothiocyanate moiety showed conjugation toamino-functionalized dideoxynucleotidetriphosphates.