A Precipitator for the Detection of Thiophilic Metals in Aqua

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• 作者：Tobin J. Dickerson ; Neal N. Reed ; James J. LaClair ; and Kim D. Janda
• 刊名：Journal of the American Chemical Society
• 出版年：2004
• 出版时间：December 22, 2004
• 年：2004
• 卷：126
• 期：50
• 页码：16582 - 16586
• 全文大小：291K
• 年卷期：v.126,no.50(December 22, 2004)
• ISSN：1520-5126

文摘

The detection of toxic metals including mercury and lead has become a vital analytical tool forenvironmental remediation and regulation of food stocks. A prevalent obstacle with the current assessmentof metal ion contamination originates from the lack of adequate assay throughput. In this context, a criticalconcern with current analyses stems from the fact that the majority of these assays are solution-based,and thus the response is highly dependent upon the assay environment. Herein, we describe a fluorescentdye-doped crystalline assay that offers convincing metal selection and provides detection comparable toconventional solution-based ligands used for the spectrofluorometric analysis of thiophilic heavy metal ions.While comparable in analytical performance to known methodologies, the formation of crystalline analytesprovides for signal amplification and, consequently, a powerful platform whose analysis is directly amenableto high-throughput video capture systems. This procedure has been tested in a variety of scenarios andshows good performance using readily available equipment, including a commercially available UniversalSerial Bus (USB) CCD camera. Furthermore, when developed in a microcapillary format, this assay iscapable of screening thousands of samples per day for the presence of subnanomolar concentrations ofHg²⁺ using a conventional fluorescence microscope.