Urinary Benzo[a]pyrene and Its Metabolites as Molecular Biomarkers of Asphalt Fume Exposure Characterized by Microflow LC Coupled to Hybrid Quadrupole Time-of-Flight Mass Spectrometry
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- 作者：Jin J. Wang ; David G. Frazer ; Samuel Stone ; Travis Goldsmith ; Brandon Law ; Amy Moseley ; Janet Simpson ; Ali Afshari ; and Daniel M. Lewis
- 刊名：Analytical Chemistry
- 出版年：2003
- 出版时间：November 1, 2003
- 年：2003
- 卷：75
- 期：21
- 页码：5953 - 5960
- 全文大小：124K
- 年卷期：v.75,no.21(November 1, 2003)
- ISSN：1520-6882
文摘
As a step to study the health effects of asphalt fumeexposure, an analytical method was developed to characterize benzo[a]pyrene and its hydroxy metabolites in theurine of asphalt fume-exposed rats. This method is basedon microflow liquid chromatography (LC) coupled tohybrid quadrupole orthogonal acceleration time-of-flightmass spectrometry (Q-TOFMS). Twenty-four female Sprague-Dawley rats were used in the experiment, with 8 ascontrols and 16 exposed to asphalt fumes in a whole-bodyinhalation chamber for 10 days (4 h/day). Generated at150 
C, the asphalt fume concentration in the animalexposure chamber ranged 76-117 mg/m3. In the urineof the asphalt fume-exposed rats, benzo[a]pyrene and itsmetabolites of 3-hydroxybenzo[a]pyrene, benzo[a]pyrene-7,8-dihydrodiol(±), and benzo[a]pyrene-7,8,9,10-tetrahydrotetrol(±) were identified, and their concentrationswere determined at 2.19 ± 0.49, 16.17 ± 0.3, 6.28 ±0.36, and 29.35 ± 0.26 ng/100 mL, respectively. Themetabolite concentrations from the controlled group,however, were either under the detection limits or at arelatively very low level (0.19 ± 0.41 ng/100 mL forbenzo[a]pyrene-7,8,9,10-tetrahydrotetrol metabolite). Theresults clearly indicate that the benzo[a]pyrene and itshydroxy metabolites were significantly elevated (p <0.001) in the urine of asphalt fume-exposed rats relativeto controls. The study also demonstrated that the combination of microflow LC separation and collision-induceddissociation leading to a characteristic fragmentationpattern by hybrid Q-TOFMS offers a distinct advantage forthe identifications and characterizations of the benzo[a]pyrene metabolites.
C, the asphalt fume concentration in the animalexposure chamber ranged 76-117 mg/m3. In the urineof the asphalt fume-exposed rats, benzo[a]pyrene and itsmetabolites of 3-hydroxybenzo[a]pyrene, benzo[a]pyrene-7,8-dihydrodiol(±), and benzo[a]pyrene-7,8,9,10-tetrahydrotetrol(±) were identified, and their concentrationswere determined at 2.19 ± 0.49, 16.17 ± 0.3, 6.28 ±0.36, and 29.35 ± 0.26 ng/100 mL, respectively. Themetabolite concentrations from the controlled group,however, were either under the detection limits or at arelatively very low level (0.19 ± 0.41 ng/100 mL forbenzo[a]pyrene-7,8,9,10-tetrahydrotetrol metabolite). Theresults clearly indicate that the benzo[a]pyrene and itshydroxy metabolites were significantly elevated (p <0.001) in the urine of asphalt fume-exposed rats relativeto controls. The study also demonstrated that the combination of microflow LC separation and collision-induceddissociation leading to a characteristic fragmentationpattern by hybrid Q-TOFMS offers a distinct advantage forthe identifications and characterizations of the benzo[a]pyrene metabolites.