Coenzyme Q10 Inhibits the Release of Glutamate in Rat Cerebrocortical Nerve Terminals by Suppression of Voltage-Dependent Calcium Influx and Mitogen-Activated Protein Kinase Signaling Pathway

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• 作者：Yi Chang ; Shu-Kuei Huang ; Su-Jane Wang
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2012
• 出版时间：December 5, 2012
• 年：2012
• 卷：60
• 期：48
• 页码：11909-11918
• 全文大小：366K
• 年卷期：v.60,no.48(December 5, 2012)
• ISSN：1520-5118

文摘

This study investigates the effects and possible mechanism of coenzyme Q10 (CoQ10) on endogenous glutamate release in the cerebral cortex nerve terminals of rats. CoQ10 inhibited the release of glutamate evoked by the K⁺ channel blocker 4-aminopyridine (4-AP). CoQ10 reduced the depolarization-induced increase in cytosolic [Ca²⁺]_c but did not alter the 4-AP-mediated depolarization. The effect of CoQ10 on evoked glutamate release was abolished by blocking the Ca_v2.2 (N-type) and Ca_v2.1 (P/Q-type) Ca²⁺ channels and mitogen-activated protein kinase kinase (MEK). In addition, CoQ10 decreased the 4-AP-induced phosphorylation of extracellular signal-regulated kinase 1 and 2 (ERK1/2) and synaptic vesicle-associated protein synapsin I, a major presynaptic substrate for ERK. Moreover, the inhibition of glutamate release by CoQ10 was strongly attenuated in mice without synapsin I. These results suggest that CoQ10 inhibits glutamate release from cortical synaptosomes in rats through the suppression of the presynaptic voltage-dependent Ca²⁺ entry and ERK/synapsin I signaling pathway.

Keywords:

qsSearchArea=searchText">CoQ10; qsSearchArea=searchText">glutamate release; qsSearchArea=searchText">cerebrocortical nerve terminals; qsSearchArea=searchText">ERK; qsSearchArea=searchText">voltage-dependent Ca²⁺ channels