Molecular Mobility of Scaffolds鈥?Biopolymers Influences Cell Growth
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- 作者：Rok Podlipec ; Selestina Gorgieva ; Darija Jura拧in ; Iztok Urban膷i膷 ; Vanja Kokol ; Janez 艩trancar
- 刊名：ACS Applied Materials & Interfaces
- 出版年：2014
- 出版时间：September 24, 2014
- 年：2014
- 卷：6
- 期：18
- 页码：15980-15990
- 全文大小：556K
- ISSN：1944-8252
文摘
Understanding biocompatibility of materials and scaffolds is one of the main challenges in the field of tissue engineering and regeneration. The complex nature of cell鈥揵iomaterial interaction requires extensive preclinical functionality testing by studying specific cell responses to different biomaterial properties, from morphology and mechanics to surface characteristics at the molecular level. Despite constant improvements, a more general picture of biocompatibility is still lacking and tailormade scaffolds are not yet available. The scope of our study was thus the investigation of the correlation of fibroblast cell growth on different gelatin scaffolds with their morphological, mechanical as well as surface molecular properties. The latter were thoroughly investigated via polymer molecular mobility studied by site-directed spin labeling and electron paramagnetic resonance spectroscopy (EPR) for the first time. Anisotropy of the rotational motion of the gelatin side chain mobility was identified as the most correlated quantity with cell growth in the first days after adhesion, while weaker correlations were found with scaffold viscoelasticity and no correlations with scaffold morphology. Namely, the scaffolds with highly mobile or unrestricted polymers identified with the cell growth being five times less efficient (Ncells = 60 卤 25 mm鈥?) as compared to cell growth on the scaffolds with considerable part of polymers with the restricted rotational motion (Ncells = 290 卤 25 mm鈥?). This suggests that molecular mobility of scaffold components could play an important role in cell response to medical devices, reflecting a new aspect of the biocompatibility concept.