文摘
Raspberries are rich in phenolic phytochemicals. To study the health benefits of raspberries, fourfresh raspberry varieties (Heritage, Kiwigold, Goldie, and Anne) were evaluated for total antioxidantand antiproliferative activities. The total amount of phenolics and flavonoids for each of the fourraspberry varieties was determined. The Heritage raspberry variety had the highest total phenoliccontent (512.7 ± 4.7 mg/100 g of raspberry) of the varieties measured followed by Kiwigold (451.1± 4.5 mg/100 g of raspberry), Goldie (427.5 ± 7.5 mg/100 g of raspberry), and Anne (359.2 ± 3.4mg/100 g of raspberry). Similarly, the Heritage raspberry variety contained the highest total flavonoids(103.4 ± 2.0 mg/100 g of raspberry) of the varieties tested, followed by Kiwigold (87.3 ± 1.8 mg/100g of raspberry), Goldie (84.2 ± 1.8 mg/100 g of raspberry), and Anne (63.5 ± 0.7 mg/100 g ofraspberry). The color of the raspberry juice correlated well to the total phenolic, flavonoid, andanthocyanin contents of the raspberry. Heritage had the highest a/b ratio and the darkest coloredjuice, and the Anne variety showed the lowest phytochemical content and the palest color. Heritageraspberry variety had the highest total antioxidant activity, followed by Kiwigold and Goldie, and theAnne raspberry variety had the lowest antioxidant activity of the varieties tested. The proliferation ofHepG2 human liver cancer cells was significantly inhibited in a dose-dependent manner after exposureto the raspberry extracts. The extract equivalent to 50 mg of Goldie, Heritage, and Kiwigold fruitinhibited the proliferation of those cells by 89.4 ± 0.1, 88 ± 0.2, and 87.6 ± 1.0%, respectively. Annehad the lowest antiproliferative activity of the varieties measured but still exhibited a significant inhibitionof 70.3± 1.2% with an extract equivalent to 50 mg of fruit. The antioxidant activity of the raspberrywas directly related to the total amount of phenolics and flavonoids found in the raspberry (p < 0.01).No relationship was found between antiproliferative activity and the total amount of phenolics/flavonoidsfound in the same raspberry (p > 0.05).Keywords: Antioxidant; cancer; cell culture; antiproliferative activity; phenolics; flavonoids; raspberry;fruit