Following the Dynamics of pH in Endosomes of Live Cells with SERS Nanosensors

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• 作者：Janina Kneipp ; Harald Kneipp ; Burghardt Wittig ; Katrin Kneipp
• 刊名：Journal of Physical Chemistry C
• 出版年：2010
• 出版时间：April 29, 2010
• 年：2010
• 卷：114
• 期：16
• 页码：7421-7426
• 全文大小：301K
• 年卷期：v.114,no.16(April 29, 2010)
• ISSN：1932-7455

文摘

The surface enhanced Raman scattering (SERS) spectrum of a reporter molecule attached to gold or silver nanostructures, which is pH-sensitive, can deliver information on the local pH in the environment of the nanostructure. Here, we demonstrate the use of a mobile SERS nanosensor made from gold nanaoaggregates and 4-mercaptobenzoic acid (pMBA) attached as a reporter for monitoring changes in local pH of the cellular compartments of living NIH/3T3 cells. We show that SERS nanosensors enable the dynamics of local pH in individual live cells to be followed at subendosomal resolution in a timeline of cellular processes. This information is of basic interest for a better understanding of a broad range of physiological and metabolic processes as well as for a number of biotechnological applications.