Hyperpolarization of Deuterated Metabolites via Remote Cross-Polarization and Dissolution Dynamic Nuclear Polarization

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• 作者：Basile Vuichoud ; Jonas Milani ; Aurélien Bornet ; Roberto Melzi ; Sami Jannin ; Geoffrey Bodenhausen
• 刊名：Journal of Physical Chemistry B
• 出版年：2014
• 出版时间：February 6, 2014
• 年：2014
• 卷：118
• 期：5
• 页码：1411-1415
• 全文大小：250K
• ISSN：1520-5207

文摘

In deuterated molecules such as [1-¹³C]pyruvate-d₃, the nuclear spin polarization of ¹³C nuclei can be enhanced by combining Hartmann鈥揌ahn cross-polarization (CP) at low temperatures (1.2 K) with dissolution dynamic nuclear polarization (D-DNP). The polarization is transferred from remote solvent protons to the ¹³C spins of interest. This allows one not only to slightly reduce build-up times but also to increase polarization levels and extend the lifetimes T₁(¹³C) of the enhanced ¹³C polarization during and after transfer from the polarizer to the NMR or MRI system. This extends time scales over which metabolic processes and chemical reactions can be monitored.