Highly Repeatable Dissolution Dynamic Nuclear Polarization for Heteronuclear NMR Metabolomics

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• 作者：Aurélien Bornet ; Mickaël Maucourt ; Catherine Deborde ; Daniel Jacob ; Jonas Milani ; Basile Vuichoud ; Xiao Ji ; Jean-Nicolas Dumez ; Annick Moing ; Geoffrey Bodenhausen ; Sami Jannin ; Patrick Giraudeau
• 刊名：Analytical Chemistry
• 出版年：2016
• 出版时间：June 21, 2016
• 年：2016
• 卷：88
• 期：12
• 页码：6179-6183
• 全文大小：275K
• 年卷期：0
• ISSN：1520-6882

文摘

At natural ¹³C abundance, metabolomics based on heteronuclear NMR is limited by sensitivity. We have recently demonstrated how hyperpolarization by dissolution dynamic nuclear polarization (D-DNP) assisted by cross-polarization (CP) provides a reliable way of enhancing the sensitivity of heteronuclear NMR in dilute mixtures of metabolites. In this Technical Note, we evaluate the precision of this experimental approach, a critical point for applications to metabolomics. The higher the repeatability, the greater the likelihood that one can detect small biologically relevant differences between samples. The average repeatability of our state-of-the-art D-DNP NMR equipment for samples of metabolomic relevance (20 mg dry weight tomato extracts) is 3.6% for signals above the limit of quantification (LOQ) and 6.4% when all the signals above the limit of detection (LOD) are taken into account. This first report on the repeatability of D-DNP highlights the compatibility of the technique with the requirements of metabolomics and confirms its potential as an analytical tool for such applications.