Heat-Transfer Resistance at Solid鈥揕iquid Interfaces: A Tool for the Detection of Single-Nucleotide Polymorphisms in DNA
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- 作者：Bart van Grinsven ; Natalie Vanden Bon ; Hannelore Strauven ; Lars Grieten ; Mohammed Murib ; Kathia L. Jim茅nez Monroy ; Stoffel D. Janssens ; Ken Haenen ; Michael J. Sch枚ning ; Veronique Vermeeren ; Marcel Ameloot ; Luc Michiels ; Ronald Thoelen ; Ward De Ceuninck ; Patrick Wagner
- 刊名：ACS Nano
- 出版年：2012
- 出版时间：March 27, 2012
- 年：2012
- 卷：6
- 期：3
- 页码：2712-2721
- 全文大小：537K
- 年卷期：v.6,no.3(March 27, 2012)
- ISSN：1936-086X
文摘
In this article, we report on the heat-transfer resistance at interfaces as a novel, denaturation-based method to detect single-nucleotide polymorphisms in DNA. We observed that a molecular brush of double-stranded DNA grafted onto synthetic diamond surfaces does not notably affect the heat-transfer resistance at the solid-to-liquid interface. In contrast to this, molecular brushes of single-stranded DNA cause, surprisingly, a substantially higher heat-transfer resistance and behave like a thermally insulating layer. This effect can be utilized to identify ds-DNA melting temperatures via the switching from low- to high heat-transfer resistance. The melting temperatures identified with this method for different DNA duplexes (29 base pairs without and with built-in mutations) correlate nicely with data calculated by modeling. The method is fast, label-free (without the need for fluorescent or radioactive markers), allows for repetitive measurements, and can also be extended toward array formats. Reference measurements by confocal fluorescence microscopy and impedance spectroscopy confirm that the switching of heat-transfer resistance upon denaturation is indeed related to the thermal on-chip denaturation of DNA.