Use of Carbonate and Alkali To Eliminate Escherichia coli from Dairy Cattle Manure
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- 作者：Francisco Diez-Gonzalez ; Graeme N. Jarvis ; David A. Adamovich ; and James B. Russell
- 刊名：Environmental Science & Technology
- 出版年：2000
- 出版时间：April 1, 2000
- 年：2000
- 卷：34
- 期：7
- 页码：1275 - 1279
- 全文大小：118K
- 年卷期：v.34,no.7(April 1, 2000)
- ISSN：1520-5851
文摘
A procedure to eliminate Escherichia coli in dairy cattlemanure was developed. E. coli persisted in fresh manure andfarm storage tanks, and viable counts ranged from 105to 108/g. If the feces to urine ratio of fresh manure wasdecreased from 2.2 to 1, E. coli did not persist for 
10 days(<10 viable cells/g), and it appeared that the urine waskilling E. coli. Fecal urease contamination produced CO2, and16% was trapped as carbonate. When urine pH wasdecreased, antimicrobial effect was lost, even if the pHwas readjusted to 8.5. When E. coli K-12 and O157:H7 weretreated with Na2CO3 (100 mM, pH 8.5, 24 h), viable cellswere not detected. The E. coli count of manure (feces tourine ratio of 2.2:1) was decreased by Na2CO3 addition (8g/kg), but pH sometimes declined and carbonate waslost. When NaOH was included (2 g/kg), Na2CO3 additionscould be decreased (4 g/kg), and treatment time was 5days. Treatment cost could be <$10 year-1 (dairy cow)-1.Water dilution (3-fold) did not diminish the effectivenessof the carbonate/alkali treatment, and viability was <10 cells/g.
10 days(<10 viable cells/g), and it appeared that the urine waskilling E. coli. Fecal urease contamination produced CO2, and16% was trapped as carbonate. When urine pH wasdecreased, antimicrobial effect was lost, even if the pHwas readjusted to 8.5. When E. coli K-12 and O157:H7 weretreated with Na2CO3 (100 mM, pH 8.5, 24 h), viable cellswere not detected. The E. coli count of manure (feces tourine ratio of 2.2:1) was decreased by Na2CO3 addition (8g/kg), but pH sometimes declined and carbonate waslost. When NaOH was included (2 g/kg), Na2CO3 additionscould be decreased (4 g/kg), and treatment time was 5days. Treatment cost could be <$10 year-1 (dairy cow)-1.Water dilution (3-fold) did not diminish the effectivenessof the carbonate/alkali treatment, and viability was <10 cells/g.