Enhancement of Viniferin Production in Vitis vinifera L. cv. Alphonse Lavall茅e Cell Suspensions by Low-Energy Ultrasound Alone and in Combination with Methyl Jasmonate
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- 作者：Anna Rita Santamaria ; Marzia Innocenti ; Nadia Mulinacci ; Fabrizio Melani ; Alessio Valletta ; Ilaria Sciandra ; Gabriella Pasqua
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2012
- 出版时间：November 7, 2012
- 年：2012
- 卷：60
- 期：44
- 页码：11135-11142
- 全文大小：343K
- 年卷期：v.60,no.44(November 7, 2012)
- ISSN：1520-5118
文摘
This study examined for the first time the effect of low-energy ultrasound (US), used alone or in combination with methyl jasmonate (MeJA), on viniferin production in cell cultures of Vitis vinifera L. cv Alphonse Lavall茅e. Cell suspensions were exposed for 2 min to US (power 30, 60, and 90 mW cm鈥?). The highest viniferin production was obtained at 30 mW cm鈥?. When sonication was performed twice, the effect on viniferin production was negligible, whereas triple sonication slightly increased production. US treatment at 30 mW cm鈥? for 5 min decreased viniferin production and induced cellular death. The combined use of MeJA and US (2 min) increased the production of 未-viniferin, the dominant stilbene, more than each elicitor used alone. These results suggest that low-energy US, alone and in combination with MeJA, can act as a physical elicitor to stimulate viniferin production in V. vinifera cell cultures.