Short Soluble Coumarin Crosslinkers for Light-Controlled Release of Cells and Proteins from Hydrogels
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- 作者：Caroline de Gracia Lux ; Jacques Lux ; Guillaume Collet ; Sha He ; Minnie Chan ; Jason Olejniczak ; Alexandra Foucault-Collet ; Adah Almutairi
- 刊名：Biomacromolecules
- 出版年：2015
- 出版时间：October 12, 2015
- 年：2015
- 卷：16
- 期：10
- 页码：3286-3296
- 全文大小：541K
- ISSN：1526-4602
文摘
Materials that degrade or dissociate in response to low power light promise to enable on-demand, precisely localized delivery of drugs or bioactive molecules in living systems. Such applications remain elusive because few materials respond to wavelengths that appreciably penetrate tissues. The photocage bromohydroxycoumarin (Bhc) is efficiently cleaved upon low-power ultraviolet (UV) and near-infrared (NIR) irradiation through one- or two-photon excitation, respectively. We have designed and synthesized a short Bhc-bearing crosslinker to create light-degradable hydrogels and nanogels. Our crosslinker breaks by intramolecular cyclization in a manner inspired by the naturally occurring ornithine lactamization, in response to UV and NIR light, enabling rapid degradation of polyacrylamide gels and release of small hydrophilic payloads such as an 鈭?0 nm model protein and murine mesenchymal stem cells, with no background leakage.