Efficient, Non-Toxic Hybrid PPV-PAMAM Dendrimer as a Gene Carrier for Neuronal Cells

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• 作者：Ana C. Rodrigo ; Iva虂n Rivilla ; Francisco C. Pe虂rez-Marti虂nez ; Silvia Monteagudo ; Vanessa Ocan虄a ; Javier Guerra ; Joaqui虂n C. Garci虂a-Marti虂nez ; Sonia Merino ; Prado Sa虂nchez-Verdu虂 ; Valenti虂n Cen虄a ; Julia虂n Rodri虂guez-Lo虂pez
• 刊名：Biomacromolecules
• 出版年：2011
• 出版时间：April 11, 2011
• 年：2011
• 卷：12
• 期：4
• 页码：1205-1213
• 全文大小：923K
• 年卷期：v.12,no.4(April 11, 2011)
• ISSN：1526-4602

文摘

A novel hybrid dendrimer (TRANSGEDEN) that combines a conjugated rigid polyphenylenevinylene (PPV) core with flexible polyamidoamine (PAMAM) branches at the surface was synthesized and characterized. The potential of this material as a nonviral gene delivery system was also examined, and it was observed that dendriplexes formed by TRANSGEDEN and small interfering ribonucleic acids (siRNAs) can be incorporated into >90% of neuronal cells without any toxicity up to a dendrimer concentration of 3 渭M. TRANSGEDEN was used to deliver a specific siRNA to rat cerebellar granular neurons (CGNs) to knock down the cofilin-1 protein. Cofilin-1 removal partially protects CGNs from N-methyl D-aspartate (NMDA)-mediated neuronal death.