Studies on the Antioxidant Activity of Pomegranate (Punica granatum) Peel and Seed Extracts Using in Vitro Models
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- 作者：R. P. Singh ; K. N. Chidambara Murthy ; and G. K. Jayaprakasha
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2002
- 出版时间：January 2, 2002
- 年：2002
- 卷：50
- 期：1
- 页码：81 - 86
- 全文大小：115K
- 年卷期：v.50,no.1(January 2, 2002)
- ISSN：1520-5118
文摘
Antioxidant-rich fractions were extracted from pomegranate (Punica granatum) peels and seeds usingethyl acetate, methanol, and water. The extracts were screened for their potential as antioxidantsusing various in vitro models, such as 
ges/gifchars/beta2.gif" BORDER=0 ALIGN="middle">-carotene-linoleate and 1,1-diphenyl-2-picryl hydrazyl (DPPH)model systems. The methanol extract of peels showed 83 and 81% antioxidant activity at 50 ppmusing the ges/gifchars/beta2.gif" BORDER=0 ALIGN="middle">-carotene-linoleate and DPPH model systems, respectively. Similarly, the methanol extractof seeds showed 22.6 and 23.2% antioxidant activity at 100 ppm using the ges/gifchars/beta2.gif" BORDER=0 ALIGN="middle">-carotene-linoleate andDPPH model systems, respectively. As the methanol extract of pomegranate peel showed the highestantioxidant activity among all of the extracts, it was selected for testing of its effect on lipid peroxidation,hydroxyl radical scavenging activity, and human low-density lipoprotein (LDL) oxidation. The methanolextract showed 56, 58, and 93.7% inhibition using the thiobarbituric acid method, hydroxyl radicalscavenging activity, and LDL oxidation, respectively, at 100 ppm. This is the first report on theantioxidant properties of the extracts from pomegranate peel and seeds. Owing to this property, thestudies can be further extended to exploit them for their possible application for the preservation offood products as well as their use as health supplements and neutraceuticals.Keywords: Punica granatum; antioxidant activity; ges/gifchars/beta2.gif" BORDER=0 ALIGN="middle">-carotene-linoleate model system; DPPH; LDL;free radicals