Photosystem I in Langmuir鈥揃lodgett and Langmuir鈥揝chaefer Monolayers

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• 作者：Xun Yan ; Christopher J. Faulkner ; G. Kane Jennings ; David E. Cliffel
• 刊名：Langmuir
• 出版年：2012
• 出版时间：October 23, 2012
• 年：2012
• 卷：28
• 期：42
• 页码：15080-15086
• 全文大小：388K
• 年卷期：v.28,no.42(October 23, 2012)
• ISSN：1520-5827

文摘

Photosystem I (PSI) is a membrane protein complex that generates photoinduced electrons and transfers them across the thylakoid membrane during photosynthesis. The PSI complex, separated from spinach leaves, was spread onto the air鈥搘ater interface as a monolayer and transferred onto a gold electrode surface that was precoated with a self-assembled monolayer (SAM). The electrochemical properties of the transferred PSI monolayer, including cyclic voltammetry and photoinduced chronoamperometry, were measured. The results showed that PSI retained its bioactivity after the manipulation. Its capability of converting photoenergy into electrical potential was demonstrated by its reducing an electron acceptor, dichloroindophenol (DCIP), and by oxidizing an electron donor, sodium ascorbate (ASC). We have shown that the protein has two possible orientations at the water interface. The orientation distribution was determined by comparing the controlled reductive and oxidative photocurrents generated from Langmuir鈥揃lodgett and Langmuir鈥揝chaefer monolayers.