Differentiation of Tonsil-Tissue-Derived Mesenchymal Stem Cells Controlled by Surface-Functionalized Microspheres in PEG-Polypeptide Thermogels

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• 作者：Eun Jeong Kye ; Seung-Jin Kim ; Min Hee Park ; Hyo Jung Moon ; Kyung Ha Ryu ; Byeongmoon Jeong
• 刊名：Biomacromolecules
• 出版年：2014
• 出版时间：June 9, 2014
• 年：2014
• 卷：15
• 期：6
• 页码：2180-2187
• 全文大小：413K
• 年卷期：v.15,no.6(June 9, 2014)
• ISSN：1526-4602

文摘

Poly(ethylene glycol)-poly(l-alanine) diblock copolymer (PEG-L-PA; molecular weight of each block of 1000鈥?080 Da) aqueous solutions undergo sol-to-gel transition in a 3.0鈥?.0 wt % concentration range as the temperature increases. By incorporating the polystyrene microspheres with different functional groups with a size of 100鈥?00 渭m in in situ formed PEG-L-PA thermogels, the differentiation of tonsil-tissue-derived mesenchymal stem cells (TMSCs) was investigated. The mRNA expression and immunohistochemical assays suggested that the TMSCs preferentially undergo adipogenesis in the ammonium (鈭扤H₃⁺)- or thiol (鈭扴H)-functionalized microsphere incorporated thermogels; chondrogenesis in the thiol-, phosphate (PO₃^{2鈥?/sup>)-, or carboxylate (鈭扖OO鈥?/sup>)-functionalized microsphere incorporated thermogels; and osteogenesis in the phosphate-, carboxylate-functionalized, or neat polystyrene microsphere incorporated thermogels. This paper provides a new TMSC 3D culture system of a sol鈥揼el reversible matrix and suggests that the surface-functional groups of microspheres in the thermogel can control the preferential differentiation of stem cells into specific cell types during the 3D culture.}