Two-Photon Fluorescent Turn-On Probe for Lipid Rafts in Live Cell and Tissue

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• 作者：Hwan Myung Kim ; Byeong Ha Jeong ; Ju-Yong Hyon ; Myoung Jin An ; Mun Sik Seo ; Jin Hee Hong ; Kyoung J. Lee ; Chul Hoon Kim ; Taiha Joo ; Seok-Cheol Hong ; Bong Rae Cho
• 刊名：Journal of the American Chemical Society
• 出版年：2008
• 出版时间：April 2, 2008
• 年：2008
• 卷：130
• 期：13
• 页码：4246 - 4247
• 全文大小：125K
• 年卷期：v.130,no.13(April 2, 2008)
• ISSN：1520-5126

文摘

We report a new two-photon fluorescence turn-on probe 6-[(E)-3-oxo-1-dodecenyl]-2-[N-methyl-N-(carboxymethyl)amino]naphthalene (CL2) that is designed specifically for visualizing lipid rafts in living cells and tissues. This probe emits much brighter two-photon excited fluorescence in lipid rafts than in non-raft domains and allows direct visualization of the lipid rafts in the live cells and pyramidal neuron layer of the CA1 region at a depth of 100-250 m in live tissues using two-photon microscopy.