RIDME Spectroscopy with Gd(III) Centers
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- 作者：Sahand Razzaghi ; Mian Qi ; Anna I. Nalepa ; Adelheid Godt ; Gunnar Jeschke ; Anton Savitsky ; Maxim Yulikov
- 刊名：The Journal of Physical Chemistry Letters
- 出版年：2014
- 出版时间：November 20, 2014
- 年：2014
- 卷：5
- 期：22
- 页码：3970-3975
- 全文大小：316K
- ISSN：1948-7185
文摘
The relaxation induced dipolar modulation enhancement (RIDME) technique is applied at W-band microwave frequencies around 94 GHz to a pair of Gd(III) complexes that are connected by a rodlike spacer, and the extraction of the interspin distance distribution is discussed. A dipolar pattern derived from RIDME experimental data is a superposition of Pake-like dipolar patterns corresponding to the fundamental dipolar interaction and higher harmonics thereof. Intriguingly, the relative weights of the stretched patterns do not depend significantly on mixing time. As much larger modulation depths can be achieved than in double electron鈥揺lectron resonance distance measurements at the same frequency, Gd(III)鈥揋d(III) RIDME may become attractive for structural characterization of biomacromolecules and biomolecular complexes.