Direct Quantitation of the Quorum Sensing Signal, Autoinducer-2, in Clinically Relevant Samples by Liquid Chromatography−Tandem Mass Spectrometry

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• 作者：Shawn R. Campagna ; Jessica R. Gooding ; Amanda L. May
• 刊名：Analytical Chemistry
• 出版年：2009
• 出版时间：August 1, 2009
• 年：2009
• 卷：81
• 期：15
• 页码：6374-6381
• 全文大小：225K
• 年卷期：v.81,no.15(August 1, 2009)
• ISSN：1520-6882

文摘

Quorum Sensing is a type of bacterial cell-to-cell signaling that allows for cell density dependent regulation of gene expression. Many of the behaviors mediated by quorum sensing are critical for bacterial colonization or infection, and autoinducer-2 has been proposed as a universal interspecies signaling molecule that allows multispecies colonies of bacteria, e.g., biofilms or dental plaque, to behave as pseudomulticellular organisms. However, the direct detection of autoinducer-2 has been difficult, leaving the in vivo relevance of this signal in question. Herein we report a liquid chromatography−tandem mass spectrometric technique that enables reproducible, quantitative, and sensitive measurement of the concentration of autoinducer-2 from a variety of sources. This technique was applied to the detection of autoinducer-2 from Escherichia coli and Vibrio harveyi in proof-of-concept studies and was then used to directly measure the concentration of the signal produced by oral bacteria in human saliva.