Fluorescence Discrimination of Cancer from Inflammation by Molecular Response to COX-2 Enzymes

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• 作者：Hua Zhang ; Jiangli Fan ; Jingyun Wang ; Bairui Dou ; Fan Zhou ; Jianfang Cao ; Junle Qu ; Zhi Cao ; Weijie Zhao ; Xiaojun Peng
• 刊名：Journal of the American Chemical Society
• 出版年：2013
• 出版时间：November 20, 2013
• 年：2013
• 卷：135
• 期：46
• 页码：17469-17475
• 全文大小：430K
• 年卷期：v.135,no.46(November 20, 2013)
• ISSN：1520-5126

文摘

Accurate identification of cancer from inflammation and normal tissue in a rapid, sensitive, and quantitative fashion is important for cancer diagnosis and resection during surgery. Here we report the use of cyclooxygenase-2 as a marker for identification of cancer from inflammation and the design of a novel smart COX-2-specific fluorogenic probe (NANQ-IMC6). The probe鈥檚 fluorescence is 鈥渢urned on鈥?in both inflammations and cancers where COX-2 is overexpressed. Intriguingly, the fluorescent emission is quite different at these two sites with different expression level of COX-2. Hence, NANQ-IMC6 can not only distinguish normal cells/tissues from cancer cells/tissues but also distinguish the latter from sites of inflammation lesions by the different fluorescence recognition of NANQ-IMC6 for COX-2 enzymes. Following spraying with the NANQ-IMC6 solution, cancerous tissue, inflamed tissues, and normal tissues can be accurately discriminated in vivo by the unaided eye using a hand-held ultraviolet lamp emitting at 365 nm. So the probe may have potential application varying from cancer inflammation diagnosis to guiding tumor resection during surgery.