Adsorption of Plasmid DNA onto N,N'- (Dimethylamino)ethyl-methacrylate Graft-Polymerized Poly-L-lactic Acid Film Surface for Promotion of in-Situ Gene Delivery

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• 作者：Tingting Jiang ; JianBin Chang ; Chunming Wang ; Zhi Ding ; Jiangning Chen ; Junfeng Zhang ; En-Tang Kang
• 刊名：Biomacromolecules
• 出版年：2007
• 出版时间：June 2007
• 年：2007
• 卷：8
• 期：6
• 页码：1951 - 1957
• 全文大小：495K
• 年卷期：v.8,no.6(June 2007)
• ISSN：1526-4602

文摘

The surface of biodegradable poly-L-lactic acid (PLLA) film was modified with N,N'-(dimethylamino)ethyl-methacrylate (DMAEMA) via UV-induced graft copolymerization, and plasmid DNA molecules were adsorbedonto the surface of modified PLLA film by electrostatic interactions with cationic DMAEMA polymer. Wecharacterized the structure of the modified PLLA film surface by Fourier transform infrared attenuated totalreflection (FTIR-ATR) spectroscopy and X-ray photoelectron spectroscopy (XPS). The weight-average molecularweight (Mw) of grafted DMAEMA polymer chains was estimated from the elution time of gel filtrationchromatography. C.I. Acid Orange 7 dyeing results indicated that graft density of DMAEMA on PLLA filmincreased with the UV irradiation time and then reached a saturated value. DNA adsorption density was proportionedto graft density of DMAEMA. Mouse fibroblast L929 cell line was cultured on modified PLLA films, and cellviability and gene transfection efficiency were monitored after 2 days culture. It was found that the DMAEMAgrafted PLLA film had obvious cytotoxicity to the cells. On the contrary, cytotoxicity of the surface was highlydecreased after adsorption with plasmid DNA. This DNA adsorbed DMAEMA modified PLLA showed the abilityto deliver DNA into mammalian cells cultured on the surface with high-transfection efficiency at a low DNAamount. The present results suggest that the DMAEMA grafted PLLA has potentiality to be used as a safe andeffective gene delivery system in gene-activated materials.