Ultrasensitive and Quantitative Detection of a New 尾-Agonist Phenylethanolamine A by a Novel Immunochromatographic Assay Based on Surface-Enhanced Raman Scattering (SERS)
详细信息 查看全文
- 作者：Mingxin Li ; Hong Yang ; Shuqun Li ; Kang Zhao ; Jianguo Li ; Danni Jiang ; Lulu Sun ; Anping Deng
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2014
- 出版时间：November 12, 2014
- 年：2014
- 卷：62
- 期：45
- 页码：10896-10902
- 全文大小：310K
- ISSN：1520-5118
文摘
Phenylethanolamine A (PA) is a new kind of 尾-agonist, which was illegally used as a feed additive for growth promotion in China. In this study, a novel immunochromatographic assay (ICA) based on surface-enhanced Raman scattering (SERS) for the ultrasensitive and quantitative detection of phenylethanolamine A is presented. The principle of this new ICA is similar to that based on colloidal gold particles, but using AuMBA@Ag-Ab [e.g., polyclonal antibody of PA labeled Au@Ag core鈥搒hell nanoparticles (NPs) sandwiched with a Raman reporter (4-mercaptobenzoic acid, MBA)] as a probe. After ICA procedures, the specific Raman scattering intensity of MBA on the test line was measured for quantitative detection of PA. This assay was completed within 15 min. The IC50 and limit of detection (LOD) values of the ICA for PA detection were 0.06 ng mL鈥? and 0.32 pg mL鈥?, respectively, which were 1鈥? orders of magnitude lower than those obtained by other immunoassays, indicating the ultrasensitivity of this ICA. There was no cross-reactivity (CR) of the assay with another three 尾-agonists (ractopamine, clenbuterol, and salbutamol), suggesting high specificity of the SERS-based ICA. A spiking experiment revealed that the recoveries of PA from pig urine samples were in range of 99.9鈥?101.2% with relative standard deviations (RSDs) of 3.6鈥?.8%. The results demonstrated that this SERS-based ICA was able to quantitatively detect PA in urine samples with high sensitivity, specificity, precision, and accuracy and might be a powerful method for the analysis of other target analytes in the food area.