Mesoporous Carbon Nanospheres Featured Fluorescent Aptasensor for Multiple Diagnosis of Cancer in Vitro and in Vivo

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• 作者：Chengyi Li ; Ying Meng ; Shanshan Wang ; Min Qian ; Jianxin Wang ; Weiyue Lu ; Rongqin Huang
• 刊名：ACS Nano
• 出版年：2015
• 出版时间：December 22, 2015
• 年：2015
• 卷：9
• 期：12
• 页码：12096-12103
• 全文大小：569K
• ISSN：1936-086X

文摘

Multiple diagnosis of cancer by a facile fluorescent sensor is extremely attractive. Herein, a Cy3-labeled ssDNA probe (P₀-Cy3) was 蟺鈥撓€ stacked on the surface of oxidized mesoporous carbon nanospheres (OMCN) to construct the fluorescent 鈥渢urn-on鈥?aptasensor. Attributing to the intrinsic properties of OMCN, the OMCN-based aptasensor not only can be used to detect mucin1 protein in liquid with a wide range of 0.1鈥?0.6 渭mol/L, a low detection limit of 6.52 nmol/L, and good selectivity, but also can quantify the cancer cells in solution with the linear range of 10⁴鈥? 脳 10⁶ cells/mL and a detection limit of 8500 cells/mL. Fascinatingly, this OMCN-based aptasensor was exploited to image cancer via solid tissues such as cells, tissue sections, and ex vivo and in vivo tumors, in which the obvious distinguishability between cancer and normal tissues was clearly demonstrated. This is a robust and simple detection technique, which can well achieve the multiple diagnosis of cancer in vitro and in vivo.